Brand

MultiSciences

Cat Num

70-EK1165

Product Name

Human BMP-7 ELISA Kit

Customs Name

Human BMP-7 ELISA Kit

Application

ELISA

Reactivity

Human

Assay Type

Sandwich ELISA

Suitable Sample Type

serum, plasma, cell culture supernates

Format

96-well strip plate

Storage

4 (unopened)standard stored at -20, others stored at 4 (opened)

Shipping Condition

4

Sample Volume

20 l

Sensitivity

2.06 pg/ml

Standard Curve Range

7.81 - 500 pg/ml

Spike Recovery Range

74 % - 120 %

Mean Spike Recovery

0.98

CV of Intra plate

4.4 % - 5.3 %

CV of Inter plate

2.3 % - 4.9 %

Components

96-well polystyrene microplate coated with a monoclonal antibody against BMP-7
Human BMP-7 Standard, lyophilized
BMP-7 Detect Antibody
Standard Diluent
Streptavidin-HRP
Assay Buffer (10)
Substrate (TMB)
Stop Solution
Washing Buffer (20)
Plate Covers

Describtion

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human BMP-7. The Human BMP-7 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Bone morphogenetic protein 7 (BMP-7) expressed in the brain, kidneys and bladder is a member of the TGF- superfamily. It plays a key role in the transformation of mesenchymal cells into bone and cartilage. BMP-7 induces the phosphorylation of SMAD1 and SMAD5, which in turn induce transcription of numerous osteogenic genes. It has been demonstrated that BMP7 treatment is sufficient to induce all of the genetic markers of osteoblast differentiation in many cell types.
BMP-7 has the potential for treatment of chronic kidney disease. BMP-7 administration has been proposed as a possible treatment for human infertility due to poor response to FSH treatment. A BMP-7 therapy for obesity in humans may be developed as a result.